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Anti-Human DLL3 Recombinant Antibody (SAA1488), FITC (HP628217)

Anti-Human DLL3 Recombinant Antibody (SAA1488), FITC
Anti-Human DLL3 Recombinant Antibody (SAA1488), FITC
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Overview
Catalog No.HP628217
Description
Anti-Human DLL3 Recombinant Antibody (SAA1488), FITC (HP628217) is a mouse monoclonal antibody detecting DLL3 in ELISA, FCM. Suitable for Human, Mouse, Rat.
Highlights
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
  • ●FITC Conjugated — Ready for direct detection without secondary antibody.
Species reactivityHuman, Mouse, Rat
ApplicationsFCM
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDSAA1488
ConjugationFITC
Tested applications FCM
Target Drosophila Delta homolog 3, Delta3, Delta-like protein 3, DLL3
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Associated products Mouse IgG2a kappa Recombinant Isotype Control Antibody (MOPC-173), FITC
Accession Q9NYJ7
Form Liquid
Storage buffer 0.01M PBS pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Delta-like protein 3 (DLL3) is a ~64 kDa protein. Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human DLL3 Recombinant Antibody (SAA1488), FITC

    Flow cytometry

    Flow-cytometry using FITC anti-human DLL3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human DLL3 monoclonal antibody (Catalog HP628217) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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