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Anti-Human ELK1 Polyclonal Antibody (HB988014)

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Overview
Catalog No.HB988014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ELK1 (Asp2-Ser87).
Target ELK1, ETS domain-containing protein Elk-1
Purification Purified by antigen affinity column.
Accession P19419
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

ETS domain-containing protein Elk-1 (ELK1) is a ~44 kDa protein. Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation.

1. Huang, Y. et al. (2000) Biochemical and biophysical research communications 271, 456-63. PMID: 10799319
2. Gille, H. et al. (1995) The EMBO journal 14, 951-62. PMID: 7889942
3. Janknecht, R. et al. (1992) Nucleic acids research 20, 3317-24. PMID: 1630903
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ × V₁ = C₂ × V₂
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human ELK1 Polyclonal Antibody

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