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Anti-Human ENPP7 Polyclonal Antibody (HD356014)

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Overview
Catalog No.HD356014
Description
Anti-Human ENPP7 Polyclonal Antibody (HD356014) is a rabbit polyclonal antibody detecting ENPP7. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ENPP7 (Asn31-Leu413).
Target Alk-SMase, Alkaline sphingomyelin phosphodiesterase, E-NPP 7, EC:3.1.4.12, ENPP7, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, Intestinal alkaline sphingomyelinase, NPP-7
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6UWV6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (ENPP7) is a ~51 kDa protein. Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. Also has phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation. Does not have nucleotide pyrophosphatase activity. May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen.

1. Duan, RD. et al. (2003) Journal of lipid research 44, 1241-50. PMID: 12671034
2. Duan, RD. et al. (2003) The Journal of biological chemistry 278, 38528-36. PMID: 12885774
3. Wu, J. et al. (2004) American journal of physiology. Gastrointestinal and liver physiology 287, G967-73. PMID: 15205117
4. Wu, J. et al. (2006) The Biochemical journal 394, 299-308. PMID: 16255717
5. Gorelik, A. et al. (2017) The Journal of biological chemistry 292, 7087-7094. PMID: 28292932
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ENPP7 Polyclonal Antibody

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