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Anti-Human ERP44 Polyclonal Antibody (HD557014)

Anti-Human ERP44 Polyclonal Antibody
Anti-Human ERP44 Polyclonal Antibody
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Overview
Catalog No.HD557014
Description
Anti-Human ERP44 Polyclonal Antibody (HD557014) is a rabbit polyclonal antibody detecting ERP44. Suitable for Mouse and Human.
Highlights
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ERP44 (Thr36-Leu406).
Target ER protein 44, ERP44, ERp44, Endoplasmic reticulum resident protein 44, KIAA0573, TXNDC4, Thioredoxin domain-containing protein 4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9BS26
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Endoplasmic reticulum resident protein 44 (ERP44) is a ~46 kDa protein. Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1A and ERO1B in the endoplasmic reticulum.

1. Anelli, T. et al. (2002) The EMBO journal 21, 835-44. PMID: 11847130
2. Anelli, T. et al. (2003) The EMBO journal 22, 5015-22. PMID: 14517240
3. Higo, T. et al. (2005) Cell 120, 85-98. PMID: 15652484
4. Zhang, J. et al. (2018) The EMBO journal 37. PMID: 29858230
Note For research use only
Images
  • Anti-Human ERP44 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ERP44 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Raji cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: K562 cell lysate
    Lane 5: 293T cell lysate
    Lane 6: Jurkat cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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