



| Catalog No. | HF916023 | ||||
|---|---|---|---|---|---|
| Species reactivity | Human | ||||
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo | ||||
| Host species | Human | ||||
| Isotype | IgG4, kappa | ||||
| Clone ID | SAA2440 | ||||
| Clonality | Monoclonal | ||||
| Target | Coagulation factor IX, EC:3.4.21.22, Christmas factor, Plasma thromboplastin component, PTC, Coagulation factor IXa light chain, Coagulation factor IXa heavy chain, F9 | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Accession | P00740 | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
| ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Background | Coagulation factor IX (F9) is a ~51 kDa protein. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. 1. de la Salle, C. et al. (1993) Nouvelle revue francaise d'hematologie 35, 473-80. PMID: 8295821 2. Suehiro, K. et al. (1989) The Journal of biological chemistry 264, 21257-65. PMID: 2592373 3. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1473-82. PMID: 20121197 4. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1465-72. PMID: 20121198 5. Rawala-Sheikh, R. et al. (1992) Blood 79, 398-405. PMID: 1730085 6. Simioni, P. et al. (2009) The New England journal of medicine 361, 1671-5. PMID: 19846852 7. Cao, Q. et al. (2025) Nature 639, 816-824. PMID: 39880037 | ||||
| Note | For research use only |

SDS-PAGE for Anti-Human F9/Factor IX Antibody (SAA2440)

Flow-cytometry using anti-human F9 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human F9 monoclonal antibody (Catalog HF916023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.




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