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Anti-Human F9/Factor IX Recombinant Antibody (SAA2440) (HF916023)

Anti-Human F9/Factor IX Recombinant Antibody (SAA2440)
Anti-Human F9/Factor IX Recombinant Antibody (SAA2440)
Anti-Human F9/Factor IX Recombinant Antibody (SAA2440)
Anti-Human F9/Factor IX Recombinant Antibody (SAA2440)
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Overview
Catalog No.HF916023
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHuman
IsotypeIgG4, kappa
Clone IDSAA2440
Clonality Monoclonal
Target Coagulation factor IX, EC:3.4.21.22, Christmas factor, Plasma thromboplastin component, PTC, Coagulation factor IXa light chain, Coagulation factor IXa heavy chain, F9
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P00740
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Coagulation factor IX (F9) is a ~51 kDa protein. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

1. de la Salle, C. et al. (1993) Nouvelle revue francaise d'hematologie 35, 473-80. PMID: 8295821
2. Suehiro, K. et al. (1989) The Journal of biological chemistry 264, 21257-65. PMID: 2592373
3. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1473-82. PMID: 20121197
4. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1465-72. PMID: 20121198
5. Rawala-Sheikh, R. et al. (1992) Blood 79, 398-405. PMID: 1730085
6. Simioni, P. et al. (2009) The New England journal of medicine 361, 1671-5. PMID: 19846852
7. Cao, Q. et al. (2025) Nature 639, 816-824. PMID: 39880037
Note For research use only
Images
  • Anti-Human F9/Factor IX Recombinant Antibody (SAA2440)

    SDS-PAGE

    SDS-PAGE for Anti-Human F9/Factor IX Antibody (SAA2440)

  • Anti-Human F9/Factor IX Recombinant Antibody (SAA2440)

    Flow cytometry

    Flow-cytometry using anti-human F9 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human F9 monoclonal antibody (Catalog HF916023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
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