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Anti-Human FGF-23 Recombinant Antibody (SAA0441) (HV625107)

Anti-Human FGF-23 Recombinant Antibody (SAA0441)
Anti-Human FGF-23 Recombinant Antibody (SAA0441)
Anti-Human FGF-23 Recombinant Antibody (SAA0441)
Anti-Human FGF-23 Recombinant Antibody (SAA0441)
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Overview
Catalog No.HV625107
Species reactivityHuman
ApplicationsELISA, FCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA0441
Clonality Monoclonal
Target Phosphatonin, FGF-23, HYPF, FGF23, Fibroblast growth factor 23, Tumor-derived hypophosphatemia-inducing factor
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q9GZV9
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Fibroblast growth factor 23 (FGF23) is a ~27 kDa protein. Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Up-regulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism.

1. Unknown (2000) Nature genetics 26, 345-8. PMID: 11062477
2. Bowe, AE. et al. (2001) Biochemical and biophysical research communications 284, 977-81. PMID: 11409890
3. Shimada, T. et al. (2004) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 19, 429-35. PMID: 15040831
4. Wang, H. et al. (2008) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 23, 939-48. PMID: 18282132
Note For research use only.
Images
  • Anti-Human FGF-23 Recombinant Antibody (SAA0441)

    Flow Cytometry

    Flow-cytometry using anti-human FGF23 antibody.FGF23 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF23 antibody monoclonal antibody (Catalog # HV625107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human FGF-23 Recombinant Antibody (SAA0441)

    Bioactivity

    Detects recombinant human FGF23(Catalog No: HV625011) in indirect ELISA

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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