Anti-Human FoxP3 Monoclonal Antibody (1A209) (HV388025)

Name: Anti-Human FoxP3 Monoclonal Antibody (1A209)
Brand: abinScience
SKU: HV388025
Price: 106 USD
Availability: InStock

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Overview
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Overview

Catalog No.	HV388025
Description	Anti-Human FOXP3 Monoclonal Antibody (1A209) [1A209] (HV388025) is a mouse monoclonal antibody detecting FOXP3 in ELISA, FCM, WB. Suitable for Human. Highlights ●Recombinant — Superior batch-to-batch reproducibility with defined sequences. ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM, WB
Host species	Mouse
Isotype	IgG1
Clone ID	1A209
Clonality	Monoclonal
Immunogen	Recombinant Human FOXP3 protein.
Tested applications	FCM
Target	Forkhead box protein P3, Scurfin, Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, FOXP3, IPEX, JM2
Purification	Protein A/G purified from cell culture supernatant.
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog # HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human FOXP3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog # HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog: HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human FOXP3 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog: HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.