Anti-Human FoxP3 Monoclonal Antibody (1A209) (HV388025)

Name: Anti-Human FoxP3 Monoclonal Antibody (1A209)
Brand: abinScience
SKU: HV388025
Price: 106 USD
Availability: InStock

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Overview

Catalog No.	HV388025
Description	Anti-Human FOXP3 Monoclonal Antibody (1A209) [1A209] (HV388025) is a mouse monoclonal antibody detecting FOXP3 in ELISA, FCM, WB. Suitable for Human. Highlights ●Recombinant — Superior batch-to-batch reproducibility with defined sequences. ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM, WB
Host species	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone ID	1A209
Immunogen	Recombinant Human FOXP3 protein.
Tested applications	FCM
Target	Forkhead box protein P3, Scurfin, Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, FOXP3, IPEX, JM2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9BZS1
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Forkhead box protein P3 (FOXP3) is a ~47 kDa protein. Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. 1. Ono, M. et al. (2007) Nature 446, 685-9. PMID: 17377532 2. Bandukwala, HS. et al. (2011) Immunity 34, 479-91. PMID: 21458306 3. Vent-Schmidt, J. et al. (2014) International reviews of immunology 33, 110-28. PMID: 23947341 4. Passerini, L. et al. (2014) International reviews of immunology 33, 129-45. PMID: 24354325 5. Ramsdell, F. et al. (2014) Nature reviews. Immunology 14, 343-9. PMID: 24722479 6. Xiao, Y. et al. (2014) Cell reports 7, 1471-1480. PMID: 24835996 7. Becher, J. et al. (2018) Developmental cell 47, 592-607.e6. PMID: 30513302 8. Yang, J. et al. (2020) EMBO reports 21, e50308. PMID: 32644293 9. McMurchy, AN. et al. (2013) Blood 121, 1265-75. PMID: 23169781 10. Bettelli, E. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 5138-43. PMID: 15790681
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog # HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human FOXP3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog # HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog: HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human FOXP3 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog: HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human FoxP3 Monoclonal Antibody (1A209).pdf

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Anti-Human FoxP3 Monoclonal Antibody (1A209) (HV388025)

Overview

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References

Datasheet

SDS