Anti-Human FZR1 Polyclonal Antibody (HP589014)

Name: Anti-Human FZR1 Polyclonal Antibody
Brand: abinScience
SKU: HP589014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP589014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human FZR1 (Ser172-Arg496).

Target

FZR, hCDH1, Fizzy-related protein homolog, Cdh1/Hct1 homolog, Fzr, CDH1, CDC20-like protein 1, KIAA1242, FYR, FZR1

Purification

Purified by antigen affinity column.

Accession

Q9UM11

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Fizzy-related protein homolog (FZR1) is a ~55 kDa protein. Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

1. Lafranchi, L. et al. (2014) The EMBO journal 33, 2860-79. PMID: 25349192

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-FZR1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: PC-3 cell lysate

References

Datasheet

Anti-Human FZR1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human FZR1 Polyclonal Antibody (HP589014)

Overview

Images

References

Datasheet

SDS

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