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Anti-Human GAPDH Monoclonal Antibody (1A201) (HY030045)

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Anti-Human GAPDH Monoclonal Antibody (1A201)
Anti-Human GAPDH Monoclonal Antibody (1A201)
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Overview
Catalog No.HY030045
Description
Anti-Human GAPDH Monoclonal Antibody (1A201) [1A201] (HY030045) is a mouse monoclonal antibody detecting GAPDH in ELISA, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Recombinant — Superior batch-to-batch reproducibility with defined sequences.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b
Clone ID1A201
Immunogen E. coli - derived recombinant Human GAPDH protein (Met1-Glu335).
Target GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase
Endotoxin level < 10 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ~36 kDa protein. Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.

1. Tisdale, EJ. (2002) The Journal of biological chemistry 277, 3334-41. PMID: 11724794
2. Ercolani, L. et al. (1988) The Journal of biological chemistry 263, 15335-41. PMID: 3170585
3. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094
4. Gao, X. et al. (2013) Cell host & microbe 13, 87-99. PMID: 23332158
5. Gao, X. et al. (2016) The Journal of biological chemistry 291, 18232-8. PMID: 27387501
Note For research use only.
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  • Anti-Human GAPDH Monoclonal Antibody (1A201)

    SDS-PAGE

    SDS-PAGE for Anti-Human GAPDH Monoclonal Antibody (1A201)

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human GAPDH Monoclonal Antibody (1A201)

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