Anti-Human GATM Polyclonal Antibody (HW668014)

Name: Anti-Human GATM Polyclonal Antibody
Brand: abinScience
SKU: HW668014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW668014

Description

Anti-Human GATM Polyclonal Antibody (HW668014) is a rabbit polyclonal antibody detecting GATM in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GATM (Glu76-Asp423).

Target

Glycine amidinotransferase, mitochondrial, L-arginine:glycine amidinotransferase, GATM, Transamidinase, AGAT

Purification

Purified by antigen affinity column.

Accession

P50440

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glycine amidinotransferase, mitochondrial (GATM) is a ~48 kDa protein. Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives. Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable).

1. Cullen, ME. et al. (2006) Circulation 114, I16-20. PMID: 16820567

2. DesRoches, CL. et al. (2016) Human mutation 37, 926-32. PMID: 27233232

3. Sinn, M. et al. (2022) Scientific reports 12, 22088. PMID: 36543883

4. Gross, MD. et al. (1986) Archives of biochemistry and biophysics 251, 747-55. PMID: 3800397

5. Fritsche, E. et al. (1997) European journal of biochemistry 247, 483-90. PMID: 9266688

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GATM1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse kidney lysate
Lane 2: Mouse pancears lysate
Lane 3: Rat kidney lysate
Lane 4: Rat pancears lysat

References

Datasheet

Anti-Human GATM Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GATM Polyclonal Antibody (HW668014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us