Human GATM Recombinant Protein (N-His) (HW668012)

Name: Human GATM Recombinant Protein (N-His)
Brand: abinScience
SKU: HW668012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW668012
Description	Recombinant Human GATM Protein, N-His (HW668012) expressed in E. coli, spanning Glu76-Asp423. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P50440
Protein length	Glu76-Asp423
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	42.57 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Glycine amidinotransferase, mitochondrial, L-arginine:glycine amidinotransferase, GATM, Transamidinase, AGAT
Background	Glycine amidinotransferase, mitochondrial (GATM) is a ~48 kDa protein. Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives. Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable). 1. Cullen, ME. et al. (2006) Circulation 114, I16-20. PMID: 16820567 2. DesRoches, CL. et al. (2016) Human mutation 37, 926-32. PMID: 27233232 3. Sinn, M. et al. (2022) Scientific reports 12, 22088. PMID: 36543883 4. Gross, MD. et al. (1986) Archives of biochemistry and biophysics 251, 747-55. PMID: 3800397 5. Fritsche, E. et al. (1997) European journal of biochemistry 247, 483-90. PMID: 9266688
Note	For research use only.

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SDS-PAGE for Recombinant Human GATM Protein, N-His

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HW668012-SDS-PAGE-1.jpg

Datasheet

Human GATM Recombinant Protein (N-His).pdf

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Human GATM Recombinant Protein (N-His) (HW668012)

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