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Anti-Human GPR25 Polyclonal Antibody (HD143014)

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Overview
Catalog No.HD143014
Description
Anti-Human GPR25 Polyclonal Antibody (HD143014) is a rabbit polyclonal antibody detecting GPR25. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GPR25 (Ala2-Val40&Ala96-Ala127&Val176-Gly201&Leu263-Thr290&Leu310-Trp361).
Target GPR25, Probable G-protein coupled receptor 25
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O00155
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

C-X-C chemokine receptor GPR25 is a ~38 kDa protein. Receptor for the C-X-C chemokine CXCL17, which plays a key role in lymphocyte homing. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as RhoA pathway. Activation by CXCL17 on lymphocytes activates the RhoA pathway, which regulates cytoskeletal dynamics and integrins, mediating lymphocyte recruitment into the respiratory, upper gastrointestinal, biliary and genito-urinary tracts.

1. Ocón, B. et al. (2024) Nature 635, 736-745. PMID: 39293486
2. Hu, WF. et al. (2025) The FEBS journal 292, 5998-6015. PMID: 40279398
Note For research use only
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Formula
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Anti-Human GPR25 Polyclonal Antibody

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