Anti-Human Granzyme B Recombinant Antibody (SAA0515) (HY574013)

Name: Anti-Human Granzyme B Recombinant Antibody (SAA0515)
Brand: abinScience
SKU: HY574013
Price: 126 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY574013

Species reactivity

Human

Applications

ELISA, WB

Host species

Mouse

Isotype

IgG1

Clone ID

SAA0515

Clonality

Monoclonal

Target

Granzyme-B, Cathepsin G-like 1, Granzyme-2, grzmB, GZMB, Fragmentin-2, Human lymphocyte protein, HLP, CTLA-1, CGL1, CTSGL1, CSPB, Lymphocyte protease, T-cell serine protease 1-3E, Granzyme B, CTLA1, GRB, C11, Cytotoxic T-lymphocyte proteinase 2, SECT

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P10144

RRID

Anti-Human GZMB Antibody (SAA0515) (abinScience Cat# HY574013, RRID:AB_3725204)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Granzyme B (GZMB) is a ~27 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis.

1. Poe, M. et al. (1991) The Journal of biological chemistry 266, 98-103. PMID: 1985927

2. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682

3. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427

4. Froelich, CJ. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 7161-71. PMID: 8258716

5. Liu, Y. et al. (2020) Science immunology 5. PMID: 31953257

6. Zhang, Z. et al. (2020) Nature 579, 415-420. PMID: 32188940

7. Yang, X. et al. (1998) The Journal of biological chemistry 273, 34278-83. PMID: 9852092

Note

For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Human GZMB Antibody (ABS0372).
SDS-PAGE

SDS-PAGE for Anti-Human GZMB Antibody (SAA0515).
Western blot

Western blot analysis was performed using anti-GZMB monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human GZMB (Catalog No: HY574012)
Lane 2: negative control