Anti-Human GZMB Recombinant Antibody (scFv17), PE (HY574227)

Name: Anti-Human GZMB Recombinant Antibody (scFv17), PE
Brand: abinScience
SKU: HY574227
Price: 290 USD
Availability: InStock

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SDS

Overview

Catalog No.	HY574227
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1
Clone ID	scFv17
Conjugation	PE
Clonality	Monoclonal
Target	Cathepsin G-like 1, Granzyme-2, GZMB, grzmB, Fragmentin-2, Human lymphocyte protein, HLP, CTLA-1, CGL1, CTSGL1, CSPB, Lymphocyte protease, T-cell serine protease 1-3E, Granzyme B, CTLA1, GRB, Granzyme-B, C11, Cytotoxic T-lymphocyte proteinase 2, SECT
Endotoxin level	Please contact with the lab for this information.
Associated products	Mouse IgG1 Recombinant Isotype Control Antibody (HyHEL-10), PE
Accession	P10144
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Granzyme B (GZMB) is a ~27 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis. 1. Poe, M. et al. (1991) The Journal of biological chemistry 266, 98-103. PMID: 1985927 2. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682 3. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427 4. Froelich, CJ. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 7161-71. PMID: 8258716 5. Liu, Y. et al. (2020) Science immunology 5. PMID: 31953257 6. Zhang, Z. et al. (2020) Nature 579, 415-420. PMID: 32188940 7. Yang, X. et al. (1998) The Journal of biological chemistry 273, 34278-83. PMID: 9852092
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using PE anti-Human GZMB antibody. BXPC3 cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-Human GZMB antibody monoclonal antibody (Catalog # HY574227 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet