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Anti-Human HCRTR2 Polyclonal Antibody (HD507014)

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Overview
Catalog No.HD507014
Description
Anti-Human HCRTR2 Polyclonal Antibody (HD507014) is a rabbit polyclonal antibody detecting HCRTR2. Suitable for Human.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human HCRTR2 (Ser2-Glu54&Glu192-Met222&Leu367-Trp444).
Target HCRTR2, Hypocretin receptor type 2, ORXR2, OX2R, OXR2, Orexin receptor type 2, Ox-2-R, Ox2-R, Ox2R
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O43614
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Orexin receptor type 2 (HCRTR2) is a ~50 kDa protein. G-protein coupled receptor that binds neuropeptides orexin-A and orexin-B, two neuropeptides derived from a common precursor, prepro-orexin. Upon neuropeptide ligand binding, HCRTR2 can couple with both G(q)/11 and G(i)/o heterotrimeric G-proteins thereby initiating distinct downstream signaling cascades. Involved in regulating the sleep-wake cycle. Contributes to central regulation of glucose homeostasis.

1. Yin, J. et al. (2016) Nature structural & molecular biology 23, 293-9. PMID: 26950369
2. Hong, C. et al. (2021) Nature communications 12, 815. PMID: 33547286
3. Yin, J. et al. (2022) Nature communications 13, 2902. PMID: 35614071
4. Sakurai, T. et al. (1998) Cell 92, 573-85. PMID: 9491897
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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