Anti-Human HEXIM1 Polyclonal Antibody (HF832014)

Name: Anti-Human HEXIM1 Polyclonal Antibody
Brand: abinScience
SKU: HF832014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HF832014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HEXIM1 (Asp258-Asp359).

Target

Protein HEXIM1, Cardiac lineage protein 1, HIS1, Menage a quatre protein 1, EDG1, Estrogen down-regulated gene 1 protein, HEXIM1, Hexamethylene bis-acetamide-inducible protein 1, MAQ1, CLP1

Purification

Purified by antigen affinity column.

Accession

O94992

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein HEXIM1 is a ~40 kDa protein. Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Core component of the 7SK RNP complex: in cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

1. Yik, JH. et al. (2003) Molecular cell 12, 971-82. PMID: 14580347

2. Michels, AA. et al. (2004) The EMBO journal 23, 2608-19. PMID: 15201869

3. Yik, JH. et al. (2005) The Journal of biological chemistry 280, 16368-76. PMID: 15713661

4. Michels, AA. et al. (2003) Molecular and cellular biology 23, 4859-69. PMID: 12832472

5. Wittmann, BM. et al. (2005) Oncogene 24, 5576-88. PMID: 15940264

6. Shimizu, N. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 8555-60. PMID: 15941832

7. Kohoutek, J. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17349-54. PMID: 17088550

8. Morchikh, M. et al. (2017) Molecular cell 67, 387-399.e5. PMID: 28712728

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-HEXIM1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

References

Datasheet

Anti-Human HEXIM1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human HEXIM1 Polyclonal Antibody (HF832014)

Overview

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References

Datasheet

SDS

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