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Anti-Human HLA-ABC Recombinant Antibody (W6/32), FITC (HM834117)

Anti-Human HLA-ABC Recombinant Antibody (W6/32), FITC
Anti-Human HLA-ABC Recombinant Antibody (W6/32), FITC
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Overview
Catalog No.HM834117
Description
Anti-Human HLA-ABC Recombinant Antibody (W6/32), FITC (HM834117) is a mouse monoclonal antibody detecting HLA-ABC,HLA-A,HLA-B,HLA-C in ELISA, FCM, ICC-Fr, WB. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
  • ●FITC Conjugated — Ready for direct detection without secondary antibody.
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDW6/32
ConjugationFITC
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Associated products Mouse IgG2a kappa Recombinant Isotype Control Antibody (MOPC-173), FITC
Form Liquid
Storage buffer 0.01M PBS pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

HLA-ABC,HLA-A,HLA-B,HLA-C is a protein.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human HLA-ABC Recombinant Antibody (W6/32), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-human HLA-A,B,C Monomorphic antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HLA-A,B,C Monomorphic monoclonal antibody (Catalog: HM834117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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