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Anti-Human HSD17B2 Polyclonal Antibody (HD429014)

Anti-Human HSD17B2 Polyclonal Antibody
Anti-Human HSD17B2 Polyclonal Antibody
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Overview
Catalog No.HD429014
Description
Anti-Human HSD17B2 Polyclonal Antibody (HD429014) is a rabbit polyclonal antibody detecting HSD17B2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human HSD17B2 (Asp81-Thr387).
Target 17-beta-HSD 2, 17-beta-hydroxysteroid dehydrogenase type 2, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, EC:1.1.1.239, EC:1.1.1.62, EDH17B2, Estradiol 17-beta-dehydrogenase 2, HSD17B2, Microsomal 17-beta-hydroxysteroid dehydrogenase, SDR9C2, Short chain dehydrogenase/reductase family 9C member 2, Testosterone 17-beta-dehydrogenase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P37059
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesP37059
Background

17-beta-hydroxysteroid dehydrogenase type 2 (HSD17B2) is a ~42 kDa protein. Catalyzes the NAD-dependent oxidation of the highly active 17beta-hydroxysteroids, such as estradiol (E2), testosterone (T), and dihydrotestosterone (DHT), to their less active forms and thus regulates the biological potency of these steroids. Oxidizes estradiol to estrone, testosterone to androstenedione, and dihydrotestosterone to 5alpha-androstan-3,17-dione. Also has 20-alpha-HSD activity.

Note For research use only
Images
  • Anti-Human HSD17B2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HSD17B2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate
    Lane 2: Mouse kidney lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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