Anti-Human HTR2B Polyclonal Antibody (HA694014)

Name: Anti-Human HTR2B Polyclonal Antibody
Brand: abinScience
SKU: HA694014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HA694014

Description

Anti-Human HTR2B Polyclonal Antibody (HA694014) is a rabbit polyclonal antibody detecting 5-hydroxytryptamine receptor 2B; 5-HT-2B; 5-HT2B; Serotonin receptor 2B; HTR2B in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human HTR2B (Leu239-Val325).

Target

5-hydroxytryptamine receptor 2B; 5-HT-2B; 5-HT2B; Serotonin receptor 2B; HTR2B

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P41595

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

5-hydroxytryptamine receptor 2B (HTR2B) is a ~54 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR2B is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways.

1. Cussac, D. et al. (2008) European journal of pharmacology 594, 32-8. PMID: 18703043

2. Wang, C. et al. (2013) Science (New York, N.Y.) 340, 610-4. PMID: 23519210

3. Choi, DS. et al. (1994) FEBS letters 352, 393-9. PMID: 7926008

4. Kursar, JD. et al. (1994) Molecular pharmacology 46, 227-34. PMID: 8078486

5. Schmuck, K. et al. (1994) FEBS letters 342, 85-90. PMID: 8143856

6. Ullmer, C. et al. (1996) British journal of pharmacology 117, 1081-8. PMID: 8882600

7. Schaerlinger, B. et al. (2003) British journal of pharmacology 140, 277-84. PMID: 12970106

8. Wacker, D. et al. (2013) Science (New York, N.Y.) 340, 615-9. PMID: 23519215

10. Wacker, D. et al. (2017) Cell 168, 377-389.e12. PMID: 28129538

Note

For research use only

Images

References

Datasheet

Anti-Human HTR2B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human HTR2B Polyclonal Antibody (HA694014)

Overview

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References

Datasheet

SDS

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