Human HTR2B Recombinant Protein (N-His-KSI) (HA694012)

Name: Human HTR2B Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HA694012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA694012
Description	Recombinant Human HTR2B Protein, N-His-KSI (HA694012) expressed in E. coli, spanning Leu239-Val325. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P41595
Protein length	Leu239-Val325
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.50 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	5-hydroxytryptamine receptor 2B; 5-HT-2B; 5-HT2B; Serotonin receptor 2B; HTR2B
Background	5-hydroxytryptamine receptor 2B is a ~54 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR2B is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. 1. Cussac, D. et al. (2008) European journal of pharmacology 594, 32-8. PMID: 18703043 2. Wang, C. et al. (2013) Science (New York, N.Y.) 340, 610-4. PMID: 23519210 3. Choi, DS. et al. (1994) FEBS letters 352, 393-9. PMID: 7926008 4. Kursar, JD. et al. (1994) Molecular pharmacology 46, 227-34. PMID: 8078486 5. Schmuck, K. et al. (1994) FEBS letters 342, 85-90. PMID: 8143856 6. Ullmer, C. et al. (1996) British journal of pharmacology 117, 1081-8. PMID: 8882600 7. Schaerlinger, B. et al. (2003) British journal of pharmacology 140, 277-84. PMID: 12970106 8. Wacker, D. et al. (2013) Science (New York, N.Y.) 340, 615-9. PMID: 23519215 10. Wacker, D. et al. (2017) Cell 168, 377-389.e12. PMID: 28129538
Note	For research use only

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SDS-PAGE for Recombinant Human HTR2B Protein, N-His-KSI

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Datasheet

Human HTR2B Recombinant Protein (N-His-KSI).pdf

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Human HTR2B Recombinant Protein (N-His-KSI) (HA694012)

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