Anti-Human IgE/IGHE Recombinant Antibody (SAA0342) (HV064023)

Name: Anti-Human IgE/IGHE Recombinant Antibody (SAA0342)
Brand: abinScience
SKU: HV064023
Price: 88 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV064023

Description

Anti-Human IgE/IGHE Recombinant Antibody (SAA0342) (HV064023) is a mouse monoclonal antibody detecting IgE/IGHE in ELISA, FCM. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, FCM

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone ID

SAA0342

Tested applications

FCM

Target

IGHE, Ig epsilon chain C region, Ig epsilon chain C region ND, Immunoglobulin heavy constant epsilon

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01854

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Immunoglobulin heavy constant epsilon (IgE/IGHE) is a ~59 kDa protein. Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain.

1. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268

2. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414

3. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170

Note

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Images

Flow Cytometry

Flow-cytometry using anti-human IgE antibody.IgE Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IgE/IGHE Antibody (SAA0342).

References

Datasheet

Anti-Human IgE/IGHE Recombinant Antibody (SAA0342).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human IgE/IGHE Recombinant Antibody (SAA0342) (HV064023)

Overview

Images

References

Datasheet

SDS