





| Catalog No. | HV064033 | ||||
|---|---|---|---|---|---|
| Species reactivity | Human | ||||
| Applications | ELISA, FCM | ||||
| Host species | Mouse | ||||
| Clonality | Monoclonal | ||||
| Isotype | IgG2a, kappa | ||||
| Clone ID | SAA0343 | ||||
| Tested applications | FCM | ||||
| Target | IGHE, Ig epsilon chain C region, Ig epsilon chain C region ND, Immunoglobulin heavy constant epsilon | ||||
| Endotoxin level | < 10 EU/mg | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Accession | P01854 | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Note | For research use only. | ||||
| Isotype Control | Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307) |

Flow-cytometry using anti-human IgE antibody.IgE Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IgE antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human IgE/IGHE Antibody (SAA0343).




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