Anti-Human IGFBP1 Polyclonal Antibody (HY471014)

Name: Anti-Human IGFBP1 Polyclonal Antibody
Brand: abinScience
SKU: HY471014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY471014

Description

Anti-Human IGFBP1 Polyclonal Antibody (HY471014) is a rabbit polyclonal antibody detecting IGFBP1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IGFBP1 (Ala26-Asn259).

Target

IGFBP-1, IGFBP1, Insulin-like growth factor-binding protein 1, Placental protein 12, IBP1, IGF-binding protein 1, IBP-1, PP12

Purification

Purified by antigen affinity column.

Accession

P08833

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Insulin-like growth factor-binding protein 1 (IGFBP1) is a ~27 kDa protein. Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner. Also plays a positive role in cell migration by interacting with integrin ITGA5:ITGB1 through its RGD motif. Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway. Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation.

1. Gleeson, LM. et al. (2001) The Journal of clinical endocrinology and metabolism 86, 2484-93. PMID: 11397844

2. Sala, A. et al. (2005) The Journal of biological chemistry 280, 29812-9. PMID: 15972819

3. Jones, JI. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 10553-7. PMID: 7504269

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IGFBP1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate

References

Datasheet

Anti-Human IGFBP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human IGFBP1 Polyclonal Antibody (HY471014)

Overview

Images

References

Datasheet

SDS

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