Anti-Human ST2/IL-33R Recombinant Antibody (STLM208) (HX139033)

Name: Anti-Human ST2/IL-33R Recombinant Antibody (STLM208)
Brand: abinScience
SKU: HX139033
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX139033

Species reactivity

Human

Applications

ELISA, FCM, WB

Host species

Human

Clonality

Monoclonal

Isotype

IgG1

Clone ID

STLM208

Tested applications

FCM

Target

T1, DER4, Interleukin-1 receptor-like 1, ST2, IL1RL1, Protein ST2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q01638

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Interleukin-1 receptor-like 1 (IL1RL1/ST2) is a ~63 kDa protein. Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages.

1. Yi, XM. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2116279119. PMID: 35238669

2. Schmitz, J. et al. (2005) Immunity 23, 479-90. PMID: 16286016

Note

For research use only.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Images

SEC-HPLC

SEC-HPLC detection for Anti-Human IL1RL1/ST2 antibody (STLM208).
Bioactivity

Detects IL1RL1/ST2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL1RL1 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1RL1 antibody monoclonal antibody (Catalog # HX139033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IL1RL1 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1RL1 antibody monoclonal antibody (Catalog # HX139033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IL1RL1/ST2 antibody (STLM208).