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Anti-Human IL2 Recombinant Antibody (SAA0370) (HX011107)

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Anti-Human IL2 Recombinant Antibody (SAA0370)

Anti-Human IL2 Recombinant Antibody (SAA0370)

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Overview

Catalog No.	HX011107
Description	Anti-Human IL2 Antibody (SAA0370) [SAA0370] (HX011107) is a mouse monoclonal antibody detecting IL2 in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Mouse
Isotype	IgG2a
Clone ID	SAA0370
Clonality	Monoclonal
Target	IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P60568
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Interleukin-2 (IL2) is a ~17 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. 1. Mingari, MC. et al. (1984) Nature 312, 641-3. PMID: 6438535 2. Wang, X. et al. (2005) Science (New York, N.Y.) 310, 1159-63. PMID: 16293754 3. Stauber, DJ. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 2788-93. PMID: 16477002 4. Miyazaki, T. et al. (1994) Science (New York, N.Y.) 266, 1045-7. PMID: 7973659 5. Kirken, RA. et al. (1995) Cytokine 7, 689-700. PMID: 8580378 6. Osinalde, N. et al. (2015) Proteomics 15, 520-31. PMID: 25142963 7. Holbrook, NJ. et al. (1984) Proceedings of the National Academy of Sciences of the United States of America 81, 1634-8. PMID: 6608729
Note	For research use only.

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Bioactivity

Detects IL2 in indirect ELISAs.

References

Datasheet

Anti-Human IL2 Recombinant Antibody (SAA0370).pdf

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Anti-Human IL2 Recombinant Antibody (SAA0370)