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Anti-Human KRT8/CK-8 Recombinant Antibody (TS1) (HY427013)

Anti-Human KRT8/CK-8 Recombinant Antibody (TS1)
Anti-Human KRT8/CK-8 Recombinant Antibody (TS1)
Anti-Human KRT8/CK-8 Recombinant Antibody (TS1)
Anti-Human KRT8/CK-8 Recombinant Antibody (TS1)
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Overview
Catalog No.HY427013
Description
Anti-Human KRT8/CK-8 Recombinant Antibody (TS1) (HY427013) is a mouse monoclonal antibody detecting KRT8/CK-8 in FCM, IF, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsFCM, IF, IHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone IDTS1
Target Type-II keratin Kb8, CYK8, Keratin-8, KRT8, Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, K8
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P05787
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Keratin, type II cytoskeletal 8 (KRT8/CK-8) is a ~53 kDa protein. Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement. Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

1. Fujiwara, S. et al. (2016) Molecular biology of the cell 27, 954-66. PMID: 26823019
Note For research use only.
Images
  • Anti-Human KRT8/CK-8 Recombinant Antibody (TS1)

    Bioactivity

    SEC-HPLC detection for Anti-Human KRT8/CK-8 Antibody (TS1).

  • Anti-Human KRT8/CK-8 Recombinant Antibody (TS1)

    SDS-PAGE

    SDS-PAGE for Anti-Human KRT8/CK-8 Antibody (TS1).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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