Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Recombinant Antibody (SAA2215) (HP610013)

Name: Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Recombinant Antibody (SAA2215)
Brand: abinScience
SKU: HP610013
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP610013

Description

Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Recombinant Antibody (SAA2215) (HP610013) is a mouse monoclonal antibody detecting Lysyl Oxidase Homolog 2/LOXL2 in ELISA, IF. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IF

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

SAA2215

Target

LOXL2, Lysyl oxidase-related protein WS9-14, Lysyl oxidase homolog 2, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9Y4K0

RRID

Anti-Human LOXL2 Antibody (SAA2215) (abinScience Cat# HP610013, RRID:AB_3729708)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Lysyl oxidase homolog 2 (LOXL2) is a ~86 kDa protein. Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription. LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2.

1. Herranz, N. et al. (2016) The FEBS journal 283, 4263-4273. PMID: 27735137

2. Iturbide, A. et al. (2015) Molecular cell 58, 755-66. PMID: 25959397

3. Peinado, H. et al. (2005) The EMBO journal 24, 3446-58. PMID: 16096638

4. Cuevas, EP. et al. (2014) Biology open 3, 129-37. PMID: 24414204

5. Millanes-Romero, A. et al. (2013) Molecular cell 52, 746-57. PMID: 24239292

6. Cuevas, EP. et al. (2017) Scientific reports 7, 44988. PMID: 28332555

Note

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

SDS-PAGE

SDS-PAGE for Anti-Human LOXL2 Antibody (SAA2215)

References

Datasheet

Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Recombinant Antibody (SAA2215).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Recombinant Antibody (SAA2215) (HP610013)

Overview

Images

References

Datasheet

SDS