Anti-Human MAP2K4 Polyclonal Antibody (HW751014)

Name: Anti-Human MAP2K4 Polyclonal Antibody
Brand: abinScience
SKU: HW751014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HW751014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAP2K4 (Asn70-Asp399).

Target

MEK 4, MEK4, SAPK kinase 1, JNKK, c-Jun N-terminal kinase kinase 1, SEK1, JNK-activating kinase 1, MAPK/ERK kinase 4, MAPKK 4, SERK1, SKK1, SAPKK-1, SAPK/ERK kinase 1, PRKMK4, MKK4, MAP kinase kinase 4, MAP2K4, Dual specificity mitogen-activated protein kinase kinase 4, SAPKK1, Stress-activated protein kinase kinase 1, JNKK1

Purification

Purified by antigen affinity column.

Accession

P45985

RRID

Anti-Human MAP2K4 Polyclonal Antibody (abinScience Cat# HW751014, RRID:AB_3730809)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4) is a ~44 kDa protein. Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MAP2K4 polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: 293T cell lysate
Lane 4: HepG2 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: K562 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse brain lysate

References

Datasheet

Anti-Human MAP2K4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MAP2K4 Polyclonal Antibody (HW751014)

Overview

Images

References

Datasheet

SDS

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