Anti-Human MCM7 Recombinant Antibody (SAA1630) (HW687013)

Name: Anti-Human MCM7 Recombinant Antibody (SAA1630)
Brand: abinScience
SKU: HW687013
Price: 196 USD
Availability: InStock

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100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW687013

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1630

Clonality

Monoclonal

Target

DNA replication licensing factor MCM7, MCM2, P1.1-MCM3, CDC47 homolog, MCM7, CDC47

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P33993

RRID

Anti-Human MCM7 Antibody (SAA1630) (abinScience Cat# HW687013, RRID:AB_3727391)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

DNA replication licensing factor MCM7 is a ~81 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

1. Ishimi, Y. et al. (2015) Journal of biochemistry 157, 561-9. PMID: 25661590

2. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

3. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

4. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

5. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

6. Ishimi, Y. (1997) The Journal of biological chemistry 272, 24508-13. PMID: 9305914

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human MCM7 Antibody (SAA1630)

References

Datasheet

Anti-Human MCM7 Recombinant Antibody (SAA1630).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human MCM7 Recombinant Antibody (SAA1630) (HW687013)

Overview

Images

References

Datasheet

SDS

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