Human MCM7 Recombinant Protein (N-His) (HW687012)

Name: Human MCM7 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW687012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW687012
Description	Recombinant Human MCM7 Protein, N-His (HW687012) expressed in E. coli, spanning Met1-Lys305. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P33993
Protein length	Met1-Lys305
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNA replication licensing factor MCM7, MCM2, P1.1-MCM3, CDC47 homolog, MCM7, CDC47
Background	DNA replication licensing factor MCM7 is a ~81 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. 1. Ishimi, Y. et al. (2015) Journal of biochemistry 157, 561-9. PMID: 25661590 2. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425 3. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004 4. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328 5. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232 6. Ishimi, Y. (1997) The Journal of biological chemistry 272, 24508-13. PMID: 9305914
Note	For research use only.

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SDS-PAGE for Recombinant Human MCM7 Protein, N-His

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Datasheet

Human MCM7 Recombinant Protein (N-His).pdf

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Human MCM7 Recombinant Protein (N-His) (HW687012)

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