Anti-Human MELK Recombinant Antibody (SAA1678) (HC349013)

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100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC349013

Description

Anti-Human MELK Antibody (SAA1678) [SAA1678] (HC349013) is a mouse monoclonal antibody detecting MELK in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1678

Clonality

Monoclonal

Target

Tyrosine-protein kinase MELK, Maternal embryonic leucine zipper kinase, Protein kinase Eg3, hMELK, MELK, Protein kinase PK38, hPK38, pEg3 kinase, KIAA0175

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q14680

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Maternal embryonic leucine zipper kinase (MELK) is a ~74 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human MELK Antibody (SAA1678)

References

Datasheet

Anti-Human MELK Recombinant Antibody (SAA1678).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MELK Recombinant Antibody (SAA1678) (HC349013)

Overview

Images

References

Datasheet

SDS

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