Anti-Human MELK Polyclonal Antibody (HC349014)

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Datasheet
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Overview

Catalog No.

HC349014

Description

Anti-Human MELK Polyclonal Antibody (HC349014) is a rabbit polyclonal antibody detecting MELK. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MELK (Met1-Phe337).

Target

EC:2.7.10.2, EC:2.7.11.1, KIAA0175, MELK, Maternal embryonic leucine zipper kinase, Protein kinase Eg3, Protein kinase PK38, Tyrosine-protein kinase MELK, hMELK, hPK38, pEg3 kinase

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q14680

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Maternal embryonic leucine zipper kinase (MELK) is a ~74 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis.

Note

For research use only

Images

References

Datasheet

Anti-Human MELK Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MELK Polyclonal Antibody (HC349014)

Overview

Images

References

Datasheet

SDS

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