Anti-Human MMP14 Recombinant Antibody (LEM-2/15) (HW299013)

Name: Anti-Human MMP14 Recombinant Antibody (LEM-2/15)
Brand: abinScience
SKU: HW299013
Price: 126 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW299013

Species reactivity

Human

Applications

Blocking, ELISA, ICC, IF, FCM, WB

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

LEM-2/15

Clonality

Monoclonal

Target

MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P50281

RRID

Anti-Human MMP14 Antibody (LEM-2/15) (abinScience Cat# HW299013, RRID:AB_3725683)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Matrix metalloproteinase-14 (MMP14) is a ~65 kDa protein. Endopeptidase that degrades various components of the extracellular matrix such as collagen. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. May be involved in actin cytoskeleton reorganization by cleaving PTK7.

1. Sato, H. et al. (1994) Nature 370, 61-5. PMID: 8015608

2. Gu, G. et al. (2012) Journal of cellular biochemistry 113, 1013-21. PMID: 22065321

3. Noda, K. et al. (2003) Investigative ophthalmology & visual science 44, 2163-70. PMID: 12714657

4. Golubkov, VS. et al. (2010) The Journal of biological chemistry 285, 35740-9. PMID: 20837484

5. Jin, G. et al. (2011) The EMBO journal 30, 2281-93. PMID: 21572390

6. Cork, SM. et al. (2012) Oncogene 31, 5144-52. PMID: 22330140

7. Chow, CFW. et al. (2022) Nature metabolism 4, 203-212. PMID: 35177851

Note

For research use only.

Images

Bioactivity

Detects MMP14 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human MMP14 Antibody (LEM-2/15).
Flow Cytometry

Flow-cytometry using anti-human MMP14 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 antibody monoclonal antibody (Catalog # HW299013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human MMP14 (Catalog No: HW299022)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human MMP14 antibody. BXPC3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 monoclonal antibody (Catalog HW299013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.