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Anti-Human MYH9 Recombinant Antibody (SAA1446) (HW778013)

Anti-Human MYH9 Recombinant Antibody (SAA1446)
Anti-Human MYH9 Recombinant Antibody (SAA1446)
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Overview
Catalog No.HW778013
Species reactivityHuman
ApplicationsELISA, IF, IP, WB
Host speciesMouse
IsotypeIgG2a
Clone IDSAA1446
Clonality Monoclonal
Target NMMHC-IIA, Non-muscle myosin heavy chain IIa, Cellular myosin heavy chain, type A, Myosin heavy chain 9, NMMHC II-a, Non-muscle myosin heavy chain A, Myosin-9, MYH9, NMMHC-A, Myosin heavy chain, non-muscle IIa
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P35579
RRID Anti-Human MYH9 Antibody (SAA1446) (abinScience Cat# HW778013, RRID:AB_3727031)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Myosin-9 (MYH9) is a ~226 kDa protein. Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis. Promotes cell motility in conjunction with S100A4. During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.

1. Li, ZH. et al. (2006) Cancer research 66, 5173-80. PMID: 16707441
2. Betapudi, V. (2010) PloS one 5, e8560. PMID: 20052411
Note For research use only.
Images
  • Anti-Human MYH9 Recombinant Antibody (SAA1446)

    SDS-PAGE

    SDS-PAGE for Anti-Human MYH9 Antibody (SAA1446)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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