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Anti-MYH9 Polyclonal Antibody (HW778014)

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Overview
Catalog No.HW778014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MYH9 (Glu1740-Glu1960).
Target NMMHC-IIA, Non-muscle myosin heavy chain IIa, Cellular myosin heavy chain, type A, Myosin heavy chain 9, NMMHC II-a, Non-muscle myosin heavy chain A, Myosin-9, MYH9, NMMHC-A, Myosin heavy chain, non-muscle IIa
Purification Purified by antigen affinity column.
Accession P35579
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Myosin-9 (MYH9) is a ~226 kDa protein. Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis. Promotes cell motility in conjunction with S100A4. During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.

1. Li, ZH. et al. (2006) Cancer research 66, 5173-80. PMID: 16707441
2. Betapudi, V. (2010) PloS one 5, e8560. PMID: 20052411
Note For research use only.
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Formula
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