Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014)

Name: Anti-Human NEU3/Sialidase-3 Polyclonal Antibody
Brand: abinScience
SKU: HP187014
Price: 96 USD
Availability: InStock

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SDS

Overview

Catalog No.

HP187014

Description

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014) is a rabbit polyclonal antibody detecting Sialidase-3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NEU3/Sialidase-3 (Met1-Asn428).

Target

N-acetyl-alpha-neuraminidase 3, NEU3, Sialidase-3, Ganglioside sialidasedis, Membrane sialidase

Purification

Purified by antigen affinity column.

Accession

Q9UQ49

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Sialidase-3 (NEU3) is a ~48 kDa protein. Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes.

1. Wada, T. et al. (1999) Biochemical and biophysical research communications 261, 21-7. PMID: 10405317

2. Monti, E. et al. (2000) The Biochemical journal 349, 343-51. PMID: 10861246

3. Wang, Y. et al. (2001) European journal of biochemistry 268, 2201-8. PMID: 11298736

4. Wang, Y. et al. (2002) The Journal of biological chemistry 277, 26252-9. PMID: 12011038

5. Yamaguchi, K. et al. (2005) The Biochemical journal 390, 85-93. PMID: 15847605

6. Albohy, A. et al. (2010) Glycobiology 20, 1127-38. PMID: 20511247

7. Rodriguez-Walker, M. et al. (2017) Scientific reports 7, 4167. PMID: 28646141

8. Wada, T. et al. (2007) Oncogene 26, 2483-90. PMID: 17334392

9. Mozzi, A. et al. (2015) Glycobiology 25, 855-68. PMID: 25922362

Note

For research use only.

Images

References

Datasheet

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014)

Overview

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References

Datasheet

SDS

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