Anti-Human NKX2-5 Polyclonal Antibody (HW502014)

Name: Anti-Human NKX2-5 Polyclonal Antibody
Brand: abinScience
SKU: HW502014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HW502014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NKX2-5 (Ser146-Gly206).

Target

NKX2E, CSX, Homeobox protein Nkx-2.5, NKX2.5, NKX2-5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E

Purification

Purified by antigen affinity column.

Accession

P52952

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Homeobox protein Nkx-2.5 (NKX2-5) is a ~34 kDa protein. Transcription factor required for the development of the heart and the spleen. During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4. May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal. Binds to the core DNA motif of NPPA promoter. Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression.

1. Koss, M. et al. (2012) Developmental cell 22, 913-26. PMID: 22560297

2. Pradhan, L. et al. (2012) Biochemistry 51, 6312-9. PMID: 22849347

3. Pradhan, L. et al. (2016) Biochemistry 55, 1702-10. PMID: 26926761

4. Samokhin, AO. et al. (2018) Science translational medicine 10. PMID: 29899023

Note

For research use only.

Images

References

Datasheet

Anti-Human NKX2-5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human NKX2-5 Polyclonal Antibody (HW502014)

Overview

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References

Datasheet

SDS

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