Anti-Human NONO Polyclonal Antibody (HS752014)

Name: Anti-Human NONO Polyclonal Antibody
Brand: abinScience
SKU: HS752014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HS752014

Description

Anti-Human NONO Polyclonal Antibody (HS752014) is a rabbit polyclonal antibody detecting NonO protein in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human NONO (Arg73-Arg256).

Target

54 kDa nuclear RNA- and DNA-binding protein, NRB54, p54nrb, NMT55, NonO protein, p54(nrb), 55 kDa nuclear protein, NONO, DNA-binding p52/p100 complex, 52 kDa subunit, Non-POU domain-containing octamer-binding protein

Purification

Purified by antigen affinity column.

Accession

Q15233

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Non-POU domain-containing octamer-binding protein (NONO) is a ~54 kDa protein. DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b.

1. Zhang, Z. et al. (2001) Cell 106, 465-75. PMID: 11525732

2. Peng, R. et al. (2002) RNA (New York, N.Y.) 8, 1334-47. PMID: 12403470

3. Mircsof, D. et al. (2015) Nature neuroscience 18, 1731-6. PMID: 26571461

8. Morchikh, M. et al. (2017) Molecular cell 67, 387-399.e5. PMID: 28712728

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NONO polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC12 cell lysate
Lane 3: 293T cell lysate