Anti-Human NOTCH2/3 Recombinant Antibody (SAA0143) (HC513107)

Name: Anti-Human NOTCH2/3 Recombinant Antibody (SAA0143)
Brand: abinScience
SKU: HC513107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HC513107
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG2, kappa
Clone ID	SAA0143
Clonality	Monoclonal
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q04721, Q9UM47
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Neurogenic locus notch homolog protein 2 (NOTCH2/NOTCH3) is a ~265 kDa protein. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. 1. Simpson, MA. et al. (2011) Nature genetics 43, 303-5. PMID: 21378985 2. Isidor, B. et al. (2011) Nature genetics 43, 306-8. PMID: 21378989 3. Fukushima, H. et al. (2017) Molecular cell 68, 645-658.e5. PMID: 29149593 4. Zhu, P. et al. (2015) Nature communications 6, 7122. PMID: 25985737
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human NOTCH2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human NOTCH3 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH3 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human NOTCH2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human NOTCH2 & NOTCH3 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 & NOTCH3 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human NOTCH2/3 Recombinant Antibody (SAA0143).pdf

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Anti-Human NOTCH2/3 Recombinant Antibody (SAA0143) (HC513107)

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