Anti-Human OPTN Polyclonal Antibody (HP451014)

Name: Anti-Human OPTN Polyclonal Antibody
Brand: abinScience
SKU: HP451014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP451014

Description

Anti-Human OPTN Polyclonal Antibody (HP451014) is a rabbit polyclonal antibody detecting OPTN in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Macaca fascicularis, and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human OPTN (Ser415-Arg524).

Target

NRP, TFIIIA-IntP, Huntingtin-interacting protein 7, HIP-7, Huntingtin yeast partner L, Transcription factor IIIA-interacting protein, NEMO-related protein, E3-14.7K-interacting protein, GLC1E, HIP7, HYPL, FIP-2, Huntingtin-interacting protein L, Optic neuropathy-inducing protein, OPTN, Optineurin, FIP2

Purification

Purified by antigen affinity column.

Accession

Q96CV9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Optineurin (OPTN) is a ~65 kDa protein. Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation. In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta/IFNB1.

1. Fifita, JA. et al. (2017) Amyotrophic lateral sclerosis & frontotemporal degeneration 18, 126-133. PMID: 27534431

2. Pourcelot, M. et al. (2016) BMC biology 14, 69. PMID: 27538435

3. Vaibhava, V. et al. (2012) Journal of cell science 125, 5026-39. PMID: 22854040

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human OPTN polyclonal antibody at 1ug/mL on recombinant Human OPTN (Catalog No. HP451012).

References

Datasheet