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Anti-Human PRMT3 Polyclonal Antibody (HT002014)

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Overview
Catalog No.HT002014
Description
Anti-Human PRMT3 Polyclonal Antibody (HT002014) is a rabbit polyclonal antibody detecting PRMT3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PRMT3 (Leu212-Gln531).
Target Protein arginine N-methyltransferase 3, 2.1.1.319, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, PRMT3, HRMT1L3
Purification Purified by antigen affinity column.
Accession O60678
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein arginine N-methyltransferase 3 (PRMT3) is a ~59 kDa protein. Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases. Catalyzes the asymmetric arginine dimethylation at multiple sites in the Arg/Gly-rich region of small ribosomal subunit protein uS5/RPS2. Also appears to methylate other ribosomal proteins. May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. Contributes to methylation of histone H4 'Arg-3', a specific tag for epigenetic transcriptional activation.

1. Siarheyeva, A. et al. (2012) Structure (London, England : 1993) 20, 1425-35. PMID: 22795084
2. Liu, F. et al. (2013) Journal of medicinal chemistry 56, 2110-24. PMID: 23445220
3. Kaniskan, HÜ. et al. (2015) Angewandte Chemie (International ed. in English) 54, 5166-70. PMID: 25728001
4. Min, Z. et al. (2019) Cell death & disease 10, 581. PMID: 31378783
5. Verma, M. et al. (2021) Communications biology 4, 109. PMID: 33495566
6. Gupta, S. et al. (2024) The Biochemical journal 481, 1723-1740. PMID: 39513743
Note For research use only.
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Formula
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Anti-Human PRMT3 Polyclonal Antibody

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