Human PRMT3 Recombinant Protein (N-His) (HT002012)

Name: Human PRMT3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT002012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT002012
Description	Recombinant Human PRMT3 Protein, N-His (HT002012) expressed in E. coli, spanning Leu212-Gln531. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60678
Protein length	Leu212-Gln531
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	38.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein arginine N-methyltransferase 3, 2.1.1.319, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, PRMT3, HRMT1L3
Background	Protein arginine N-methyltransferase 3 (PRMT3) is a ~59 kDa protein. Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases. Catalyzes the asymmetric arginine dimethylation at multiple sites in the Arg/Gly-rich region of small ribosomal subunit protein uS5/RPS2. Also appears to methylate other ribosomal proteins. May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. Contributes to methylation of histone H4 'Arg-3', a specific tag for epigenetic transcriptional activation. 1. Siarheyeva, A. et al. (2012) Structure (London, England : 1993) 20, 1425-35. PMID: 22795084 2. Liu, F. et al. (2013) Journal of medicinal chemistry 56, 2110-24. PMID: 23445220 3. Kaniskan, HÜ. et al. (2015) Angewandte Chemie (International ed. in English) 54, 5166-70. PMID: 25728001 4. Min, Z. et al. (2019) Cell death & disease 10, 581. PMID: 31378783 5. Verma, M. et al. (2021) Communications biology 4, 109. PMID: 33495566 6. Gupta, S. et al. (2024) The Biochemical journal 481, 1723-1740. PMID: 39513743
Note	For research use only.

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SDS-PAGE for Recombinant Human PRMT3 Protein, N-His

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Datasheet

Human PRMT3 Recombinant Protein (N-His).pdf

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Human PRMT3 Recombinant Protein (N-His) (HT002012)

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