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Anti-Human RDH12 Polyclonal Antibody (HA495014)

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Overview
Catalog No.HA495014
Description
Anti-Human RDH12 Polyclonal Antibody (HA495014) is a rabbit polyclonal antibody detecting RDH12 in ELISA, IHC, WB. Suitable for Bovine, Mus musculus, and Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RDH12 (Thr33-Glu316).
Target RDH12
Purification Purified by antigen affinity column.
Accession Q96NR8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesRetinol dehydrogenase 12, 1.1.1.300, All-trans and 9-cis retinol dehydrogenase, Short chain dehydrogenase/reductase family 7C member 2, RDH12, SDR7C2
Background

Retinol dehydrogenase 12 (RDH12) is a ~35 kDa protein. Retinoids dehydrogenase/reductase with a clear preference for NADP. Displays high activity towards 9-cis, 11-cis and all-trans-retinal. Shows very weak activity towards 13-cis-retinol. Also exhibits activity, albeit with lower affinity than for retinaldehydes, towards lipid peroxidation products (C9 aldehydes) such as 4-hydroxynonenal and trans-2-nonenal. May play an important function in photoreceptor cells to detoxify 4-hydroxynonenal and potentially other toxic aldehyde products resulting from lipid peroxidation.

1. Haeseleer, F. et al. (2002) The Journal of biological chemistry 277, 45537-45546. PMID: 12226107
2. Belyaeva, OV. et al. (2005) Biochemistry 44, 7035-47. PMID: 15865448
3. Marchette, LD. et al. (2010) Free radical biology & medicine 48, 16-25. PMID: 19686838
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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