Human RDH12 Recombinant Protein (N-His) (HA495012)

Name: Human RDH12 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA495012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA495012
Description	Recombinant Human RDH12 Protein, N-His (HA495012) expressed in E. coli, spanning Thr33-Glu316. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96NR8
Protein length	Thr33-Glu316
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.83 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Retinol dehydrogenase 12, 1.1.1.300, All-trans and 9-cis retinol dehydrogenase, Short chain dehydrogenase/reductase family 7C member 2, RDH12, SDR7C2
Background	Retinol dehydrogenase 12 is a ~35 kDa protein. Retinoids dehydrogenase/reductase with a clear preference for NADP. Displays high activity towards 9-cis, 11-cis and all-trans-retinal. Shows very weak activity towards 13-cis-retinol. Also exhibits activity, albeit with lower affinity than for retinaldehydes, towards lipid peroxidation products (C9 aldehydes) such as 4-hydroxynonenal and trans-2-nonenal. May play an important function in photoreceptor cells to detoxify 4-hydroxynonenal and potentially other toxic aldehyde products resulting from lipid peroxidation. 1. Haeseleer, F. et al. (2002) The Journal of biological chemistry 277, 45537-45546. PMID: 12226107 2. Belyaeva, OV. et al. (2005) Biochemistry 44, 7035-47. PMID: 15865448 3. Marchette, LD. et al. (2010) Free radical biology & medicine 48, 16-25. PMID: 19686838
Note	For research use only.

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SDS-PAGE for Recombinant Human RDH12 Protein, N-His

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Datasheet

Human RDH12 Recombinant Protein (N-His).pdf

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Human RDH12 Recombinant Protein (N-His) (HA495012)

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