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Anti-Human SASH1 Polyclonal Antibody (HF745014)

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Overview
Catalog No.HF745014
Description
Anti-Human SASH1 Polyclonal Antibody (HF745014) is a rabbit polyclonal antibody detecting SASH1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SASH1 (Val1091-Met1247).
Target SAM and SH3 domain-containing protein 1, Proline-glutamate repeat-containing protein, SASH1, KIAA0790, PEPE1
Purification Purified by antigen affinity column.
Accession O94885
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

SAM and SH3 domain-containing protein 1 (SASH1) is a ~136 kDa protein. Is a positive regulator of NF-kappa-B signaling downstream of TLR4 activation. It acts as a scaffold molecule to assemble a molecular complex that includes TRAF6, MAP3K7, CHUK and IKBKB, thereby facilitating NF-kappa-B signaling activation. Regulates TRAF6 and MAP3K7 ubiquitination. Involved in the regulation of cell mobility. Regulates lipolysaccharide (LPS)-induced endothelial cell migration.

1. Dauphinee, SM. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 892-901. PMID: 23776175
2. Zhou, D. et al. (2013) Cellular signalling 25, 1526-38. PMID: 23333244
3. Courcet, JB. et al. (2015) European journal of human genetics : EJHG 23, 957-62. PMID: 25315659
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SASH1 Polyclonal Antibody

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