Human SASH1 Recombinant Protein (N-His) (HF745012)

Name: Human SASH1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF745012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HF745012
Description	Recombinant Human SASH1 Protein, N-His (HF745012) expressed in E. coli, spanning Val1091-Met1247. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O94885
Protein length	Val1091-Met1247
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.54 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SAM and SH3 domain-containing protein 1, Proline-glutamate repeat-containing protein, SASH1, KIAA0790, PEPE1
Background	SAM and SH3 domain-containing protein 1 (SASH1) is a ~136 kDa protein. Is a positive regulator of NF-kappa-B signaling downstream of TLR4 activation. It acts as a scaffold molecule to assemble a molecular complex that includes TRAF6, MAP3K7, CHUK and IKBKB, thereby facilitating NF-kappa-B signaling activation. Regulates TRAF6 and MAP3K7 ubiquitination. Involved in the regulation of cell mobility. Regulates lipolysaccharide (LPS)-induced endothelial cell migration. 1. Dauphinee, SM. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 892-901. PMID: 23776175 2. Zhou, D. et al. (2013) Cellular signalling 25, 1526-38. PMID: 23333244 3. Courcet, JB. et al. (2015) European journal of human genetics : EJHG 23, 957-62. PMID: 25315659
Note	For research use only.

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SDS-PAGE for Recombinant Human SASH1 Protein, N-His

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Datasheet

Human SASH1 Recombinant Protein (N-His).pdf

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Human SASH1 Recombinant Protein (N-His) (HF745012)

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