Anti-Human Serpin E1/PAI-1 Recombinant Antibody (8H9D4) (HY185023)

Name: Anti-Human Serpin E1/PAI-1 Recombinant Antibody (8H9D4)
Brand: abinScience
SKU: HY185023
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY185023

Brand

abinScience

Description

Anti-Human Serpin E1/PAI-1 Recombinant Antibody (8H9D4) (HY185023) is a mouse monoclonal antibody detecting Serpin E1/PAI-1 in ELISA, Neutralization, WB. Suitable for Human, Porcine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Porcine

Applications

ELISA, Neutralization, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a

Clone ID

8H9D4

Target

PLANH1, Endothelial plasminogen activator inhibitor, SERPINE1, Plasminogen activator inhibitor 1, PAI1, PAI, PAI-1, Serpin E1

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P05121

RRID

Anti-Human SERPINE1/PAI-1 Antibody (8H9D4) (abinScience Cat# HY185023, RRID:AB_3726308)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Plasminogen activator inhibitor 1 (SERPINE1/PAI-1) is a ~45 kDa protein. Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading.

1. Szabo, R. et al. (2005) The Biochemical journal 390, 231-42. PMID: 15853774

2. Jankun, J. et al. (2007) International journal of molecular medicine 20, 683-7. PMID: 17912461

3. Lee, MH. et al. (1993) Blood 81, 2357-62. PMID: 8481516

4. Fay, WP. et al. (1997) Blood 90, 204-8. PMID: 9207454

5. Anisuzzaman et al. (2011) Biochemical and biophysical research communications 413, 599-604. PMID: 21925150

6. Planus, E. et al. (1997) Journal of cell science 110 ( Pt 9), 1091-8. PMID: 9175705

7. Degryse, B. et al. (2004) The Journal of biological chemistry 279, 22595-604. PMID: 15001579

8. Kjøller, L. et al. (1997) Experimental cell research 232, 420-9. PMID: 9168821

9. Providence, KM. et al. (2008) Archives of dermatological research 300, 303-10. PMID: 18386027

10. Kortlever, RM. et al. (2006) Nature cell biology 8, 877-84. PMID: 16862142

Note

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

SDS-PAGE

SDS-PAGE for Anti-Human SERPINE1/PAI-1 Antibody (8H9D4)

References

Datasheet

Anti-Human Serpin E1/PAI-1 Recombinant Antibody (8H9D4).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Serpin E1/PAI-1 Recombinant Antibody (8H9D4) (HY185023)

Overview

Images

References

Datasheet

SDS