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Anti-Human SLC5A4 Polyclonal Antibody (HP385014)

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Overview
Catalog No.HP385014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SLC5A4 (Thr548-Arg637).
Target SAAT1, Solute carrier family 5 member 4, SLC5A4, SGLT3
Purification Purified by antigen affinity column.
Accession Q9NY91
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Probable glucose sensor protein SLC5A4 is a ~72 kDa protein. Does not function as sodium/D-glucose symporter. However, may function as a D-glucose sensor by generating a D-glucose-induced depolarization which is pH-independent, Na(+)-dependent at neutral pH and probably H(+)-dependent at acidic pH.

1. Diez-Sampedro, A. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 11753-8. PMID: 13130073
2. Bianchi, L. et al. (2010) PloS one 5, e10241. PMID: 20421923
3. Kothinti, RK. et al. (2012) European journal of pharmacology 690, 77-83. PMID: 22766068
4. Voss, AA. et al. (2007) Molecular pharmacology 71, 628-34. PMID: 17110502
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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