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Human SLC5A4 Recombinant Protein (N-GST & C-His) (HP385022)

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Overview
Catalog No.HP385022
Description
Recombinant Human SLC5A4 Protein, N-GST & C-His (HP385022) expressed in E. coli, spanning Thr548-Arg637. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ9NY91
Protein lengthThr548-Arg637
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 38.68 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesProbable glucose sensor protein SLC5A4, SAAT1, SGLT3, SLC5A4, Solute carrier family 5 member 4
Background

Probable glucose sensor protein SLC5A4 is a ~72 kDa protein. Does not function as sodium/D-glucose symporter. However, may function as a D-glucose sensor by generating a D-glucose-induced depolarization which is pH-independent, Na(+)-dependent at neutral pH and probably H(+)-dependent at acidic pH.

1. Diez-Sampedro, A. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 11753-8. PMID: 13130073
2. Bianchi, L. et al. (2010) PloS one 5, e10241. PMID: 20421923
3. Kothinti, RK. et al. (2012) European journal of pharmacology 690, 77-83. PMID: 22766068
4. Voss, AA. et al. (2007) Molecular pharmacology 71, 628-34. PMID: 17110502
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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