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Anti-Human TAF5 Polyclonal Antibody (HD242014)

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Overview
Catalog No.HD242014
Description
Anti-Human TAF5 Polyclonal Antibody (HD242014) is a rabbit polyclonal antibody detecting TAF5. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TAF5 (Leu458-Gln800).
Target TAF(II)100, TAF2D, TAF5, TAFII-100, TAFII100, Transcription initiation factor TFIID 100 kDa subunit, Transcription initiation factor TFIID subunit 5
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q15542
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Transcription initiation factor TFIID subunit 5 (TAF5) is a ~86 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TAF5 is involved in two modules of TFIID, in TFIID-A together with TAF3 and TBP, and in TFIID-B with TAF8.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Dubrovskaya, V. et al. (1996) The EMBO journal 15, 3702-12. PMID: 8758937
3. Tanese, N. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 13611-6. PMID: 8942982
4. Tao, Y. et al. (1997) The Journal of biological chemistry 272, 6714-21. PMID: 9045704
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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Anti-Human TAF5 Polyclonal Antibody

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